beta_profile_region.py
=======================

Description
------------
This program calculates methylation profile (i.e. average beta value) around the user-specified genomic regions.

**Example of input**

::
 
 # BED6 format (INPUT_FILE)
 chr22   44021512        44021513        cg24055475      0.9231  -
 chr13   111568382       111568383       cg06540715      0.1071  +
 chr20   44033594        44033595        cg21482942      0.6122  -
 
 # BED3 format (REGION_FILE)
 chr1    15864   15865
 chr1    18826   18827
 chr1    29406   29407

Options
-----------

  --version             show program's version number and exit
  -h, --help            show this help message and exit
  -i INPUT_FILE, --input_file=INPUT_FILE
                        BED6+ file specifying the C position. This BED file
                        should have at least six columns (Chrom, ChromStart,
                        ChromeEnd, Name, Beta_value, Strand). BED6+ file can
                        be a regular text file or compressed file (.gz, .bz2).
  -r REGION_FILE, --region=REGION_FILE
                        BED3+ file of genomic regions. This BED file should
                        have at least three columns (Chrom, ChromStart,
                        ChromeEnd). If the 6-th column does not exist, all
                        regions will be considered as on "+" strand.
  -d DOWNSTREAM_SIZE, --downstream=DOWNSTREAM_SIZE
                        Size of extension to downstream. default=2000 (bp)
  -u UPSTREAM_SIZE, --upstream=UPSTREAM_SIZE
                        Size of extension to upstream. default=2000 (bp)
  -o OUT_FILE, --output=OUT_FILE
                        The prefix of the output file.

Input files (examples)
--------------------------
- `test_02.bed6.gz <https://sourceforge.net/projects/cpgtools/files/test/test_02.bed6.gz>`_
- `hg19.RefSeq.union.1Kpromoter.bed <https://sourceforge.net/projects/cpgtools/files/test/hg19.RefSeq.union.1Kpromoter.bed.gz/download>`_


Command
-----------
::

 $beta_profile_region.py -r hg19.RefSeq.union.1Kpromoter.bed.gz -i test_02.bed6.gz -o region_profile

Output files
---------------

- region_profile.txt
- region_profile.r
- region_profile.pdf

.. image:: ../_static/region_profile.png
   :height: 400 px
   :width: 500 px
   :scale: 100 %